J1300
1300 .
TEPIDANAEROBACTER GT38 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1300 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 2.90 | g | 2.9 | 16.65 | |
| KH2PO4 | 1.50 | g | 1.5 | 11.022 | |
| Urea | 2.10 | g | 2.1 | 34.967 | |
| Yeast extract | 6.00 | g | 6 | - | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Na2CO3 | 4.00 | g | 4 | 37.74 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
| Mineral solution | 0.20 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Mix components thoroughly. Distribute the medium in culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with CO2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere): | |||||
|
Glucose
(w/v, 10%) |
100.00 | ml | - | - | |
| 2 Adjust pH to 7.0, if necessary. | |||||
Solution occurs in 3
other media (first defined in Medium J1274).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| MgCl2 x 6 H2O | 25.000 | g | 25 | 122.969 |
| CaCl2 x 2 H2O | 37.500 | g | 37.5 | 255.076 |
| FeSO4 x 7 H2O | 0.312 | g | 0.312 | 1.122 |
| Distilled water | 1000.000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |