# J1300: TEPIDANAEROBACTER GT38 MEDIUM


## Main sol. J1300

2.9	g	K2HPO4	
1.5	g	KH2PO4	
2.1	g	Urea	
6	g	Yeast extract	
0.01	g	CaCl2 x 2 H2O	
4	g	Na2CO3	
0.5	g	L-Cysteine HCl x H2O	
0.5	mg	Resazurin	
0.2	ml	**Mineral solution**	
900	ml	Distilled water	

1. Mix components thoroughly. Distribute the medium in culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with CO2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere): 

100	ml	Glucose (w/v, 10%)	

2. Adjust pH to 7.0, if necessary.


## Mineral solution

25	g	MgCl2 x 6 H2O	
37.5	g	CaCl2 x 2 H2O	
0.312	g	FeSO4 x 7 H2O	
1000	ml	Distilled water