Main sol. J1300
Internal Solution-ID: S5456
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 2.90 | g | 2.9 | 16.65 | |
| KH2PO4 | 1.50 | g | 1.5 | 11.022 | |
| Urea | 2.10 | g | 2.1 | 34.967 | |
| Yeast extract | 6.00 | g | 6 | - | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Na2CO3 | 4.00 | g | 4 | 37.74 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
| Mineral solution | 0.20 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Mix components thoroughly. Distribute the medium in culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with CO2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere): | |||||
|
Glucose
(w/v, 10%) |
100.00 | ml | - | - | |
| 2 Adjust pH to 7.0, if necessary. | |||||
Media with this solution
| J1300 | TEPIDANAEROBACTER GT38 MEDIUM |