J1299
1299 .
PAENIBACILLUS DC-8A MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1299 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| 1 Mix components thoroughly and adjust pH to 9.0. Distribute the medium into culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 2.90 | g | 2.9 | 16.65 | |
| Urea | 4.20 | g | 4.2 | 69.935 | |
| Yeast extract | 2.00 | g | 2 | - | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Na2CO3 | 1.00 | g | 1 | 9.435 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
| Mineral solution | 0.20 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
|
Xylose
(w/v, 10%) |
100.00 | ml | - | - | |
Solution occurs in 3
other media (first defined in Medium J1274).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| MgCl2 x 6 H2O | 25.000 | g | 25 | 122.969 |
| CaCl2 x 2 H2O | 37.500 | g | 37.5 | 255.076 |
| FeSO4 x 7 H2O | 0.312 | g | 0.312 | 1.122 |
| Distilled water | 1000.000 | ml | - | - |
Metadata
| Last modified: | 27.03.23 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |