1299 .
PAENIBACILLUS DC-8A MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
9.0
Select a strain (optional):
ml
| 1 Mix components thoroughly and adjust pH to 9.0. Distribute the medium into culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere): | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 2.90 | g | ||
|
|
Urea | 4.20 | g | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.01 | g | ||
|
|
Na2CO3 | 1.00 | g | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
Resazurin | 0.50 | mg | ||
|
|
Mineral solution | 0.20 | ml | ||
|
|
Distilled water | 900.00 | ml | ||
|
|
Xylose
(w/v, 10%) |
100.00 | ml | ||
Mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgCl2 x 6 H2O | 25.000 | g |
|
|
CaCl2 x 2 H2O | 37.500 | g |
|
|
FeSO4 x 7 H2O | 0.312 | g |
|
|
Distilled water | 1000.000 | ml |