J1198
1198 .
SALTWATER MEDIUM FOR OCEANIC AOA
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1198 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.0 | g | 26 | 444.901 | |
| MgSO4 x 7 H2O | 5.0 | g | 5 | 20.286 | |
| MgCl2 x 6 H2O | 5.0 | g | 5 | 24.594 | |
| CaCl2 x 2 H2O | 1.5 | g | 1.5 | 10.203 | |
| KBr | 0.1 | g | 0.1 | 0.84 | |
| Distilled water | 980.0 | ml | - | - | |
| 1 Mix components thoroughly and autoclave. After cooling, aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order: | |||||
| HEPES solution | 10.0 | ml | - | - | |
|
KH2PO4
(3 mM) |
5.0 | ml | - | - | |
|
NaHCO3
(*, 1 M) |
2.0 | ml | - | - | |
| Modified trace element mixture | 1.0 | ml | - | - | |
|
FeNa-EDTA
(pH 7.0, *, 7.5 mM) |
1.0 | ml | - | - | |
|
NH4Cl
(1 M) |
1.0 | ml | - | - | |
| 2 Check pH of the medium to be 7.2. Prior to use, add per liter 1.0 ml catalase solution (5-10 units/ml, filter-sterilized, freshly prepared) to the medium. | |||||
| 3 Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking. | |||||
Solution occurs in 1
other media (first defined in Medium J1004).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaOH | 12.0 | g | - | - | |
|
HEPES
(free acid) |
119.2 | g | - | - | |
| 1 Dissolve NaOH in 300 ml distilled water. While stirring vigorously, slowly add HEPES until completely dissolved. Fill up to 450 ml with distilled water. If 1 ml of this solution is added to 100ml of the medium (containing all additive but HEPES), pH should be around 7.6 at 30C. Otherwise, adjust pH of the HEPES solution with 10 N NaOH or conc. HCl. Finally fill up to 500 ml with distilled water. | |||||
Solution occurs in 1
other media (first defined in Medium J1004).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 26 | 444.901 |
| MgCl2 x 6 H2O | 5 | 24.594 |
| MgSO4 x 7 H2O | 5 | 20.286 |
| CaCl2 x 2 H2O | 1.5 | 10.203 |
| KBr | 0.1 | 0.84 |
| Show more | ||
