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JCM 1004 . FRESHWATER MEDIUM FOR SOIL AOA

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Basic FWM solution #

1017
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.0 g 0.983 16.826
KCl 0.5 g 0.492 6.595
MgCl2 x 6 H2O 0.4 g 0.393 1.935
KH2PO4 0.2 g 0.197 1.445
CaCl2 x 2 H2O 0.1 g 0.098 0.669
Distilled water 1000.0 ml - -
1 Autoclave Basic FWM solution and aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order:
Modified trace element mixture 1.0 ml - -
Vitamin solution
(*) 		1.0 ml - -
FeNa-EDTA
(pH 7.0, *, 7.5 mM) 		1.0 ml - -
NaHCO3
(*, 1 M) 		2.0 ml - -
HEPES solution 10.0 ml - -
NH4Cl
(1 M) 		1.0 ml - -
Sodium pyruvate
(*, 1 M) 		1.0 ml - -
2 Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking.

Modified trace element mixture #

1000
Solution occurs in 1 other media (first defined in Medium J1004).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		12.5 ml - -
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 -
Thiamine HCl 50 mg 0.05 -
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
DL-Calcium pantothenate 50 mg 0.05 0.105
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
Choline chloride 2 g 2 14.325
Distilled water 1000 ml - -
1 Adjust pH to 7.0 with KOH.

HEPES solution #

Solution occurs in 1 other media (first defined in Medium J1004).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 12.0 g - -
HEPES
(free acid) 		119.2 g - -
1 Dissolve NaOH in 300 ml distilled water. While stirring vigorously, slowly add HEPES until completely dissolved. Fill up to 450 ml with distilled water. If 1 ml of this solution is added to 100ml of the medium (containing all additive but HEPES), pH should be around 7.6 at 30C. Otherwise, adjust pH of the HEPES solution with 10 N NaOH or conc. HCl. Finally fill up to 500 ml with distilled water.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Cultivation metadata from 2 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
nutrition chemolithotroph 1 strain
oxygen aerobe 1 strain
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