HEPES solution
Internal Solution-ID: S5044
This solution is used in 2 media and was first defined in medium J1004.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaOH | 12.0 | g | - | - | |
|
HEPES
(free acid) |
119.2 | g | - | - | |
| 1 Dissolve NaOH in 300 ml distilled water. While stirring vigorously, slowly add HEPES until completely dissolved. Fill up to 450 ml with distilled water. If 1 ml of this solution is added to 100ml of the medium (containing all additive but HEPES), pH should be around 7.6 at 30C. Otherwise, adjust pH of the HEPES solution with 10 N NaOH or conc. HCl. Finally fill up to 500 ml with distilled water. | |||||