Basic FWM solution
Internal Solution-ID: S5041
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.0 | g | 0.983 | 16.826 | |
| KCl | 0.5 | g | 0.492 | 6.595 | |
| MgCl2 x 6 H2O | 0.4 | g | 0.393 | 1.935 | |
| KH2PO4 | 0.2 | g | 0.197 | 1.445 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.098 | 0.669 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave Basic FWM solution and aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order: | |||||
| Modified trace element mixture | 1.0 | ml | - | - | |
|
Vitamin solution
(*) |
1.0 | ml | - | - | |
|
FeNa-EDTA
(pH 7.0, *, 7.5 mM) |
1.0 | ml | - | - | |
|
NaHCO3
(*, 1 M) |
2.0 | ml | - | - | |
| HEPES solution | 10.0 | ml | - | - | |
|
NH4Cl
(1 M) |
1.0 | ml | - | - | |
|
Sodium pyruvate
(*, 1 M) |
1.0 | ml | - | - | |
| 2 Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking. | |||||
Media with this solution
| J1004 | FRESHWATER MEDIUM FOR SOIL AOA |