Main sol. J1198
Internal Solution-ID: S5317
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.0 | g | 26 | 444.901 | |
| MgSO4 x 7 H2O | 5.0 | g | 5 | 20.286 | |
| MgCl2 x 6 H2O | 5.0 | g | 5 | 24.594 | |
| CaCl2 x 2 H2O | 1.5 | g | 1.5 | 10.203 | |
| KBr | 0.1 | g | 0.1 | 0.84 | |
| Distilled water | 980.0 | ml | - | - | |
| 1 Mix components thoroughly and autoclave. After cooling, aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order: | |||||
| HEPES solution | 10.0 | ml | - | - | |
|
KH2PO4
(3 mM) |
5.0 | ml | - | - | |
|
NaHCO3
(*, 1 M) |
2.0 | ml | - | - | |
| Modified trace element mixture | 1.0 | ml | - | - | |
|
FeNa-EDTA
(pH 7.0, *, 7.5 mM) |
1.0 | ml | - | - | |
|
NH4Cl
(1 M) |
1.0 | ml | - | - | |
| 2 Check pH of the medium to be 7.2. Prior to use, add per liter 1.0 ml catalase solution (5-10 units/ml, filter-sterilized, freshly prepared) to the medium. | |||||
| 3 Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking. | |||||
Media with this solution
| J1198 | SALTWATER MEDIUM FOR OCEANIC AOA |