C66
66 .
NCL/0.01%NPA
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C66 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NCL (New Cereal Leaf-Prescott Liquid) | fill up to 1000 | ml | - | - | |
| 0.01% NPA (New Cereal Leaf-Prescott Agar) | fill up to 1000 | ml | - | - | |
| 1 Biphasic. See separate recipes. | |||||
Solution occurs in 1
other media (first defined in Medium C66).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| modified NCL (New Cereal Leaf-Prescott Liquid) | 1000 | ml | - | - | |
|
Agar
(Oxoid L11) |
15 | g | 15 | - | |
| 1 Make up 1 litre of New Cereal Leaf-Prescott Liquid (NCL - see separate recipe), but use only 0.1 g of cereal leaves. Mix with 15 g of Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 1
other media (first defined in Medium C66).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium C76 | 1000.0 | ml | - | - | |
|
Cereal leaves
(Cerophyll) |
0.1 | g | 0.1 | - | |
| Deionized water | 1000.0 | ml | - | - | |
| 1 Bring PJ (Mail sol. C 76) to the boil then add cereal leaves. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 13
other media (first defined in Medium C76).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Stock solution (CaCl2/KCl) | 1 | ml | - | - | |
|
K2HPO4
(0.51 g/100 ml stock solution) |
1 | ml | - | - | |
|
MgSO4 x 7 H2O
(0.28 g/100 ml stock solution) |
1 | ml | - | - | |
| Deionized water | 1000 | ml | - | - | |
| 1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 3
other media (first defined in Medium C64).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium C76 | 1000 | ml | - | - | |
|
Cereal leaves
(Cerophyll) |
1 | g | 1 | - | |
| Deionized water | 1000 | ml | - | - | |
| 1 Bring PJ (Mail sol. C 76) to the boil then add cereal leaves. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes. | |||||
Solution occurs in 13
other media (first defined in Medium C76).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.43 | g | 4.3 | 29.249 |
| KCl | 0.16 | g | 1.6 | 21.462 |
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |