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modified NCL (New Cereal Leaf-­Prescott Liquid)

Internal Solution-ID: S6210

This solution is used in 2 media and was first defined in medium C66.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium C76 1000.0 ml - -
Cereal leaves
0.1 g 0.1 -
Deionized water 1000.0 ml - -
1 Bring PJ (Mail sol. C 76) to the boil then add cereal leaves. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.
C66 NCL/0.01%NPA
C67 NCL:PJ/0.01%NPA