# C66: NCL/0.01%NPA


## Mail sol. C66

x	ml	**NCL (New Cereal Leaf-­Prescott Liquid)**	
x	ml	**0.01% NPA (New Cereal Leaf-­Prescott Agar)**	

1. Biphasic.  See separate recipes.


## 0.01% NPA (New Cereal Leaf-­Prescott Agar)

1000	ml	**modified NCL (New Cereal Leaf-­Prescott Liquid)**	
15	g	Agar (Oxoid L11)	

1. Make up 1 litre of New Cereal Leaf-­Prescott Liquid (NCL ­- see separate recipe), but
use only 0.1 g of cereal leaves.  Mix with 15 g of Bacteriological Agar (Oxoid L11).
Autoclave at 15 psi for 15 minutes.


## modified NCL (New Cereal Leaf-­Prescott Liquid)

1000	ml	**Mail sol. C76**	
0.1	g	Cereal leaves (Cerophyll)	
1000	ml	Deionized water	

1. Bring PJ (Mail sol. C 76) to the boil then add cereal leaves.  Continue to boil for 5 minutes.  Allow to
cool then restore volume to 1 litre with deionized water.  Filter through GF/C paper 
and autoclave at 15 psi for 15 minutes.


## Mail sol. C76

1	ml	**Stock solution (CaCl2/KCl)**	
1	ml	K2HPO4 (0.51 g/100 ml stock solution)	
1	ml	MgSO4 x 7 H2O (0.28 g/100 ml stock solution)	
1000	ml	Deionized water	

1. Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.


## NCL (New Cereal Leaf-­Prescott Liquid)

1000	ml	**Mail sol. C76**	
1	g	Cereal leaves (Cerophyll)	
1000	ml	Deionized water	

1. Bring PJ (Mail sol. C 76) to the boil then add cereal leaves.  Continue to boil for 5 minutes.  Allow to
cool then restore volume to 1 litre with deionized water.  Filter through GF/C paper 
and autoclave at 15 psi for 15 minutes.


## Stock solution (CaCl2/KCl)

0.43	g	CaCl2 x 2 H2O	
0.16	g	KCl