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DSMZ

DSMZ 950 . ONR7a MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Gemmobacter megaterium DSM 26375

* Modification: Add 1% sodium pyruvate

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C

Main sol. 950 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 500 ml - -
Solution 2 450 ml - -
Solution 3 50 ml - -
Sodium pyruvate* 10 g/l - -
1 Autoclave the solutions separately. All solutions should be mixed after autoclaving when they have cooled to at least 50°C. For solid media add to the first solution 15 g/l of Bacto agar or 12 g/l of agarose (Sigma).

Solution 1 #

500
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 22.79 g 45.58 779.945
Na2SO4 3.98 g 7.96 56.041
KCl 0.72 g 1.44 19.316
NaBr 83.00 mg 0.166 1.613
NaHCO3 31.00 mg 0.062 0.738
H3BO3 27.00 mg 0.054 0.873
NaF 2.60 mg 0.005 0.124
NH4Cl 0.27 g 0.54 10.095
Na2HPO4 x 7 H2O 89.00 mg 0.178 1.254
TAPSO 1.30 g 2.6 10.028
Distilled water 500.00 ml - -
Agar , for solid medium, alternative 15.00 g 30 89.198
Agarose , for solid medium, alternative 12.00 g 24 38.065
1 adjust pH to 7.6 with NaOH

Solution 2 #

450
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 11.18 g 24.844 122.204
CaCl2 x 2 H2O 1.46 g 3.244 22.069
SrCl2 x 6 H2O 24.00 mg 0.053 0.2
Distilled water 450.00 ml - -

Solution 3 #

50
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 2 mg 0.04 0.201
Distilled water 50 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.6
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 8
Strains with modifications: 8
Gemmobacter megaterium DSM 26375

  10.0 g/l Sodium pyruvate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
NaCl 22.79 389.973
Agar 15 44.599
Agarose 12 19.033
MgCl2 x 6 H2O 11.18 54.992
Sodium pyruvate 10 90.876
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Cultivation metadata from 8 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 8 strains
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
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