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DSMZ

DSMZ 938 . ST21 AGAR FOR CELLULOSE DEGRADERS

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Sorangium cellulosum DSM 14732

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 938 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 600 ml - -
Solution B 300 ml - -
Solution C 100 ml - -
1 Autoclave solutions separately and combine after cooling to 50°C. A slight precipitate does not restrict agar quality. Adjust pH to 7.2 with KOH using pH indicating paper strips before pouring plates. Place 3-4 strips of filter paper (ca 1 cm2) on the gelated plates and inoculate the filter strips.

Solution A #

600
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 1 g 1.667 9.569
Yeast extract
(Difco) 		20 mg 0.033 -
Agar 14 g 23.333 69.376
Tap water 600 ml - -

Solution B #

300
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 1.0 g 3.333 32.97
MgSO4 x 7 H2O 1.0 g 3.333 13.524
CaCl2 x 2 H2O 1.0 g 3.333 22.673
MnSO4 x 7 H2O 0.1 g 0.333 2.207
Tap water 300.0 ml - -

Solution C #

100
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 0.2 g 2 12.331
Tap water OR Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 7
Sorangium cellulosum DSM 14732

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 14 41.626
K2HPO4 1 5.741
KNO3 1 9.891
CaCl2 x 2 H2O 1 6.802
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 7 strains BacDive

phyla 1 7 strains
temperature mesophilic 7 strains