830
830 .
R2A MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Deinococcus antarcticus DSM 27864
* Modification: Plus vitamin solution (2ml/L); see medium 141
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C
Main sol. 830 #
ml
Solution occurs in 9
other media (first defined in Medium 830).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.50 | g | - | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | - | - | |
| Casamino acids | 0.50 | g | - | - | |
| Glucose | 0.50 | g | - | - | |
|
Starch
(soluble) |
0.50 | g | - | - | |
| Na-pyruvate | 0.30 | g | - | - | |
| K2HPO4 | 0.30 | g | - | - | |
| MgSO4 x 7 H2O | 0.05 | g | - | - | |
| Agar | 15.00 | g | - | - | |
| Wolin's vitamin solution* | 2.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.5 | 2.775 |
| Casamino acids | 0.5 | - |
| Starch | 0.5 | - |
| Proteose peptone | 0.5 | - |
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Cultivation metadata from 1844 strains
| halophily | non-halophilic | 15 strains |
| halophily | moderately halophilic | 2 strains |
| pH | 6 - 8 | 489 strains |
| pH | 4 - 6 | 264 strains |
| pH | 8 - 10 | 242 strains |
| Show more | ||