MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 69 . STARKEYA NOVELLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 69 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 50 ml - -
Solution C 5 ml - -
Solution D 50 ml - -
1 Solutions A, B, C and D are sterilized separately by autoclaving and mixed when cooled to room temperature. Adjust pH of complete medium to 8.5 with sterile 0.5 N NaOH.

Solution A #

902
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 10.6 g 11.752 82.782
KH2PO4 1.5 g 1.663 12.22
NH4Cl 0.3 g 0.333 6.218
Yeast extract 0.3 g 0.333 -
Phenol red
(0.1% w/v) 		2.0 ml 0.002 0.006
Agar , for solid medium
(Bacto)
optional 		15.0 g 16.63 -
Distilled water 900.0 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.1 g 2 8.114
Distilled water 50.0 ml - -

Solution C #

5
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution (Vishniac & Santer, 1957) 5 ml - -

Solution D #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 100 402.924
Distilled water 50 ml - -

Trace element solution (Vishniac & Santer, 1957) #

1000
Solution occurs in 4 other media (first defined in Medium 69).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Metadata

Last modified: 20.10.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.892 -
Na2HPO4 x 12 H2O 10.524 74.133
Na2S2O3 x 5 H2O 4.975 20.046
KH2PO4 1.489 10.943
NH4Cl 0.298 5.568
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains