69 .
STARKEYA NOVELLA MEDIUM
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Equipment needed:
Autoclave
Final pH:
8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 900 | ml | ||
|
|
Solution B | 50 | ml | ||
|
|
Solution C | 5 | ml | ||
|
|
Solution D | 50 | ml | ||
| 1 Solutions A, B, C and D are sterilized separately by autoclaving and mixed when cooled to room temperature. Adjust pH of complete medium to 8.5 with sterile 0.5 N NaOH. | |||||
Solution A #
902
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2HPO4 x 12 H2O | 10.6 | g |
|
|
KH2PO4 | 1.5 | g |
|
|
NH4Cl | 0.3 | g |
|
|
Yeast extract | 0.3 | g |
|
|
Phenol red
(0.1% w/v) |
2.0 | ml |
|
|
Agar
, for solid medium
(Bacto) optional |
15.0 | g |
|
|
Distilled water | 900.0 | ml |
Trace element solution (Vishniac & Santer, 1957) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
|
|
ZnSO4 x 7 H2O | 22.00 | g | ||
|
|
CaCl2 x 2 H2O | 5.54 | g | ||
|
|
MnCl2 x 4 H2O | 5.06 | g | ||
|
|
FeSO4 x 7 H2O | 5.00 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 1.10 | g | ||
|
|
CuSO4 x 5 H2O | 1.57 | g | ||
|
|
CoCl2 x 6 H2O | 1.61 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH. | |||||