This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Medium 461 | 1000 | ml | - | - | |
Quinoline solution | 100 | ml | - | - | |
1 Prepare an emulsion of 3 g/l quinoline in 50 mM phosphate buffer by stirring or ultrasonication. Autoclave in a gas tight vessel. Add 100 ml of this emulsion to 1 l autoclaved medium 461. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Distilled water | 1000.00 | ml | - | - | |
Na2HPO4 x 2 H2O | 1.45 | g | 1.441 | 10.153 | |
KH2PO4 | 0.25 | g | 0.249 | 1.826 | |
CaCl2 | 0.01 | g | 0.01 | 0.09 | |
MgSO4 x 7 H2O | 0.50 | g | 0.497 | 2.017 | |
MnSO4 | 0.01 | g | 0.01 | 0.066 | |
NH4Cl | 0.30 | g | 0.298 | 5.575 | |
NaCl | 0.05 | g | 0.05 | 0.85 | |
Vitamin solution | 5.00 | ml | - | - | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
1 Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5. | |||||
2 Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Quinoline | 3 | g/l | 3 | 23.227 |
Phosphate buffer | 50 | mM | - | 50 |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
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All strains for this medium: | 3 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2HPO4 x 2 H2O | 1.31 | 9.23 |
MgSO4 x 7 H2O | 0.452 | 1.833 |
Quinoline | 0.273 | 2.112 |
NH4Cl | 0.271 | 5.068 |
KH2PO4 | 0.226 | 1.66 |
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