Main sol. 461
Internal Solution-ID: S925
This solution is used in 4 media and was first defined in medium 461.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Distilled water | 1000.00 | ml | - | - | |
| Na2HPO4 x 2 H2O | 1.45 | g | 1.441 | 10.153 | |
| KH2PO4 | 0.25 | g | 0.249 | 1.826 | |
| CaCl2 | 0.01 | g | 0.01 | 0.09 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.497 | 2.017 | |
| MnSO4 | 0.01 | g | 0.01 | 0.066 | |
| NH4Cl | 0.30 | g | 0.298 | 5.575 | |
| NaCl | 0.05 | g | 0.05 | 0.85 | |
| Vitamin solution | 5.00 | ml | - | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| 1 Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5. | |||||
| 2 Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source. | |||||