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DSMZ

DSMZ 461c . MINERAL MEDIUM WITH O-NITROPHENOL

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 461c #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 461 800 ml - -
Nitrophenol solution 200 ml - -
1 Prepare and autoclave medium 461 (vitamins not necessary). Add 200 ml nitrophenol solution to 800 ml medium 461.

Medium 461 #

1000
Solution occurs in 3 other media (first defined in Medium 461).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.00 ml - -
Na2HPO4 x 2 H2O 1.45 g 1.441 10.153
KH2PO4 0.25 g 0.249 1.826
CaCl2 0.01 g 0.01 0.09
MgSO4 x 7 H2O 0.50 g 0.497 2.017
MnSO4 0.01 g 0.01 0.066
NH4Cl 0.30 g 0.298 5.575
NaCl 0.05 g 0.05 0.85
Vitamin solution 5.00 ml - -
Trace element solution SL-10 1.00 ml - -
1 Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5.
2 Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source.

Nitrophenol solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
2-Nitrophenol 0.5 g 0.5 3.594
Phosphate buffer
(50 mM, pH 7.5) 		1000.0 ml - 50
1 Dissolve 0.5 g o-nitrophenol per liter phosphate buffer (50 mM, pH 7.5) and sterilize by filtration.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.153 8.123
MgSO4 x 7 H2O 0.398 1.613
NH4Cl 0.239 4.46
KH2PO4 0.199 1.461
2-Nitrophenol 0.1 0.719
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain