# 461a: MINERAL MEDIUM WITH QUINOLINE


## Main sol. 461a

1000	ml	**Main sol. 461**	
100	ml	**Quinoline solution**	

1. Prepare an emulsion of 3 g/l quinoline in 50 mM phosphate buffer by stirring or ultrasonication. Autoclave in a gas tight vessel. Add 100 ml of this emulsion to 1 l autoclaved medium 461.


## Main sol. 461

1000	ml	Distilled water	
1.45	g	Na2HPO4 x 2 H2O	
0.25	g	KH2PO4	
0.01	g	CaCl2	
0.5	g	MgSO4 x 7 H2O	
0.01	g	MnSO4	
0.3	g	NH4Cl	
0.05	g	NaCl	
5	ml	**Vitamin solution**	
1	ml	**Trace element solution SL-10**	

1. Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5.
2. Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source.


## Quinoline solution

3	g/l	Quinoline	
50	mM	Phosphate buffer