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THIOBACILLUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Thiobacillus thioparus DSM 505
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
26 °C
Main sol. 36 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.757 | |
| K2HPO4 | 4.00 | g | 4 | 22.965 | |
| KH2PO4 | 4.00 | g | 4 | 29.393 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
| CaCl2 | 0.10 | g | 0.1 | 0.901 | |
| FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.074 | |
| MnSO4 x H2O | 0.02 | g | 0.02 | 0.118 | |
|
Agar
, for solid medium
optional |
12.00 | g | 12 | 35.679 | |
| Na2S2O3 x 5 H2O | 10.00 | g | 10 | 40.292 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable culivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. | |||||
Metadata
| Last modified: | 31.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.6 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thiobacillus thioparus DSM 505
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
|
Agar optional |
12 | 35.679 |
| Na2S2O3 x 5 H2O | 10 | 40.292 |
| KH2PO4 | 4 | 29.393 |
| K2HPO4 | 4 | 22.965 |
| CaCl2 | 0.1 | 0.901 |
| Show more | ||
