36 .
THIOBACILLUS MEDIUM
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Selected strain: Thiobacillus thioparus DSM 505
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
26 °C
Equipment needed:
Autoclave
Final pH:
6.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 0.10 | g | ||
|
|
K2HPO4 | 4.00 | g | ||
|
|
KH2PO4 | 4.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 | 0.10 | g | ||
|
|
FeCl3 x 6 H2O | 0.02 | g | ||
|
|
MnSO4 x H2O | 0.02 | g | ||
|
|
Agar
, for solid medium
optional |
12.00 | g | ||
|
|
Na2S2O3 x 5 H2O | 10.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable culivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. | |||||