{"medium":{"id":36,"name":"THIOBACILLUS MEDIUM","complex_medium":"no","min_pH":6.6,"max_pH":6.6,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium36.pdf"},"solutions":[{"id":56,"name":"Main sol. 36","volume":1000,"recipe":[{"recipe No.":1,"compound":"(NH4)2SO4","compound_id":27,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.756773,"optional":"no"},{"recipe No.":2,"compound":"K2HPO4","compound_id":10,"amount":4,"unit":"g","g_l":4,"mmol_l":22.9653,"optional":"no"},{"recipe No.":3,"compound":"KH2PO4","compound_id":11,"amount":4,"unit":"g","g_l":4,"mmol_l":29.3934,"optional":"no"},{"recipe No.":4,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.405719,"optional":"no"},{"recipe No.":5,"compound":"CaCl2","compound_id":81,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.901063,"optional":"no"},{"recipe No.":6,"compound":"FeCl3 x 6 H2O","compound_id":82,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.073993,"optional":"no"},{"recipe No.":7,"compound":"MnSO4 x H2O","compound_id":14,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.118332,"optional":"no"},{"recipe No.":8,"compound":"Agar","compound_id":3,"condition":"for solid medium","attribute":"","amount":12,"unit":"g","g_l":12,"mmol_l":35.6792,"optional":"yes"},{"recipe No.":9,"compound":"Na2S2O3 x 5 H2O","compound_id":65,"amount":10,"unit":"g","g_l":10,"mmol_l":40.2924,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable culivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution."}],"equipment":{"2853":"Autoclave"},"main solution":"yes"}]}