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Main sol. 36

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Internal Solution-ID: S56

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.10 g 0.1 0.757
K2HPO4 4.00 g 4 22.965
KH2PO4 4.00 g 4 29.393
MgSO4 x 7 H2O 0.10 g 0.1 0.406
CaCl2 0.10 g 0.1 0.901
FeCl3 x 6 H2O 0.02 g 0.02 0.074
MnSO4 x H2O 0.02 g 0.02 0.118
Agar , for solid medium
optional 		12.00 g 12 35.679
Na2S2O3 x 5 H2O 10.00 g 10 40.292
Distilled water 1000.00 ml - -
1 Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable culivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution.
36 THIOBACILLUS MEDIUM