1800
1800 .
Vulcanimicrobium alpinus medium
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1800 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.44 | g | 0.44 | 3.233 | |
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.757 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
|
Yeast extract
, can be increased to 0.3% = 3g/L (Bacto™ Yeast Extract recommended) |
0.30 | g | 0.3 | - | |
| Peptone | 0.50 | g | 0.5 | - | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| Glucose | 1.00 | g/l | 1 | 5.551 | |
| Trace element solution SL-8 | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 6.0. | |||||
| 2 aliquot 5 mL medium into 50 mL serum bottles | |||||
| 3 add 10% CO2 to gasphase (air) | |||||
| 4 Autoclave at 121 °C for 15 min. | |||||
| 5 add vitamins solutions from 1000 x stocks | |||||
| Vitamin solution A (1000 x stock) | 0.25 | ml/l | - | - | |
| Vitamin solution B (1000 x stock) | 0.25 | ml/l | - | - | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 10 | mg | 0.1 | 0.409 | |
| Riboflavin | 10 | mg | 0.1 | 0.266 | |
| Thiamine HCl | 100 | mg | 1 | 2.965 | |
| Thiamine pyrophosphate | 100 | mg | 1 | 2.17 | |
| L-Ascorbic acid | 100 | mg | 1 | 5.678 | |
| Calcium pantothenate | 100 | mg | 1 | 2.098 | |
| Folic acid | 100 | mg | 1 | 2.266 | |
| Nicotinamide | 100 | mg | 1 | 8.189 | |
| Nicotinic acid | 100 | mg | 1 | 8.123 | |
| p-Aminobenzoic acid | 100 | mg | 1 | 7.292 | |
| Pyridoxine hydrochloride | 100 | mg | 1 | 4.863 | |
| Lipoic acid | 100 | mg | 1 | 4.847 | |
| beta-NAD | 100 | mg | 1 | 1.505 | |
|
Potassium phosphate buffer
(pH 7.2, 10 mM) |
100 | ml | - | 1 | |
| 1 Dissolve the vitamins in 10mM phosphate buffer, pH7.2. | |||||
| 2 Titrate with NaOH until vitamins are dissolved, filter sterilize afterwards, and freeze in appropriate aliquots until usage. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 100 | mg | 1 | 0.738 | |
| Distilled water | 100 | ml | - | - | |
| 1 Dissolve the vitamin B12 in water. | |||||
| 2 Titrate with HCl until vitamin B12 is dissolved, filter sterilize afterwards, and freeze in appropriate aliquots until usage. | |||||
Solution occurs in 2
other media (first defined in Medium 745).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 62.0 | mg | 0.062 | 1.003 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoSO4 x 2 H2O | 36.0 | mg | 0.036 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 6.8 | |||||
Metadata
| Last modified: | 05.06.24 |
| Source: | MediaDive: DSMZ |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Autoclave |
|
Description:
BasalYE Medium supplemented with yeast extract, peptone, CaCl2x2H2O and Vitamines |
|
| Edit in Medium builder | |
Gas composition
- 90 % Air
- 10 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |