Vitamin solution A (1000 x stock)
Internal Solution-ID: S5932
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 10 | mg | 0.1 | 0.409 | |
| Riboflavin | 10 | mg | 0.1 | 0.266 | |
| Thiamine HCl | 100 | mg | 1 | 2.965 | |
| Thiamine pyrophosphate | 100 | mg | 1 | 2.17 | |
| L-Ascorbic acid | 100 | mg | 1 | 5.678 | |
| Calcium pantothenate | 100 | mg | 1 | 2.098 | |
| Folic acid | 100 | mg | 1 | 2.266 | |
| Nicotinamide | 100 | mg | 1 | 8.189 | |
| Nicotinic acid | 100 | mg | 1 | 8.123 | |
| p-Aminobenzoic acid | 100 | mg | 1 | 7.292 | |
| Pyridoxine hydrochloride | 100 | mg | 1 | 4.863 | |
| Lipoic acid | 100 | mg | 1 | 4.847 | |
| beta-NAD | 100 | mg | 1 | 1.505 | |
|
Potassium phosphate buffer
(pH 7.2, 10 mM) |
100 | ml | - | 1 | |
| 1 Dissolve the vitamins in 10mM phosphate buffer, pH7.2. | |||||
| 2 Titrate with NaOH until vitamins are dissolved, filter sterilize afterwards, and freeze in appropriate aliquots until usage. | |||||
Media with this solution
| 1800 | Vulcanimicrobium alpinus medium |