Name: GEMMATIMONAS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1646 #

Equipment needed: Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract	0.25	g	0.25	-
Peptone (Difco)	0.50	g	0.5	-
Glucose	0.10	g	0.1	0.555
HEPES	1.95	g	1.95	8.183
MgSO₄ x 7 H₂O	0.37	g	0.37	1.501
CaSO₄ x 2 H₂O	0.43	g	0.43	2.498
Agar	14.00	g	14	41.626
Distilled water	1000.00	ml	-	-
Vitamin solution	5.00	ml	-	-
1 Adjust pH to 7.0 prior to the addition of agar.
2 After autoclaving and cooling to about 55°C, add 5 ml of vitamin solution.

Vitamin solution #

1000

Solution occurs in 15 other media (first defined in Medium 461).

Equipment needed: Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Vitamin B₁₂	50	mg	0.05	0.037
Pantothenic acid	50	mg	0.05	0.228
Riboflavin	50	mg	0.05	0.133
Pyridoxamine hydrochloride	10	mg	0.01	0.049
Biotin	20	mg	0.02	0.082
Folic acid	20	mg	0.02	0.045
Nicotinic acid	25	mg	0.025	0.203
Nicotine amide	25	mg	0.025	0.205
alpha-lipoic acid	50	mg	0.05	0.242
p-Aminobenzoic acid	50	mg	0.05	0.365
Thiamine-HCl x 2 H₂O	50	mg	0.05	0.134
Distilled water	1000	ml	-	-
1 Stir for some hours, filter sterilize the solution.

All strains for this medium:

Compound	Conc. [g/L]	Conc. [mM]
Agar	14	41.626
HEPES	1.95	8.183
Peptone	0.5	-
CaSO₄ x 2 H₂O	0.43	2.498
MgSO₄ x 7 H₂O	0.37	1.501
Yeast extract	0.25	-
Glucose	0.1	0.555
p-Aminobenzoic acid	2.5e-4	0.002
alpha-lipoic acid	2.5e-4	0.001
Pantothenic acid	2.5e-4	0.001
Thiamine-HCl x 2 H₂O	2.5e-4	6.7e-4
Riboflavin	2.5e-4	6.6e-4
Vitamin B₁₂	2.5e-4	1.8e-4
Nicotine amide	1.3e-4	0.001
Nicotinic acid	1.3e-4	0.001
Biotin	1.0e-4	4.1e-4
Folic acid	1.0e-4	2.3e-4
Pyridoxamine hydrochloride	5.0e-5	2.4e-4
Distilled water	-	-
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