1646 .
GEMMATIMONAS MEDIUM
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Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.25 | g | ||
|
|
Peptone
(Difco) |
0.50 | g | ||
|
|
Glucose | 0.10 | g | ||
|
|
HEPES | 1.95 | g | ||
|
|
MgSO4 x 7 H2O | 0.37 | g | ||
|
|
CaSO4 x 2 H2O | 0.43 | g | ||
|
|
Agar | 14.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Vitamin solution | 5.00 | ml | ||
| 1 Adjust pH to 7.0 prior to the addition of agar. | |||||
| 2 After autoclaving and cooling to about 55°C, add 5 ml of vitamin solution. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 50 | mg | ||
|
|
Pantothenic acid | 50 | mg | ||
|
|
Riboflavin | 50 | mg | ||
|
|
Pyridoxamine hydrochloride | 10 | mg | ||
|
|
Biotin | 20 | mg | ||
|
|
Folic acid | 20 | mg | ||
|
|
Nicotinic acid | 25 | mg | ||
|
|
Nicotine amide | 25 | mg | ||
|
|
alpha-lipoic acid | 50 | mg | ||
|
|
p-Aminobenzoic acid | 50 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 50 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Stir for some hours, filter sterilize the solution. | |||||