147
147 .
RUMINOBACTER AMYLOPHILUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 147 #
ml
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.45 | g | 0.45 | 2.584 | |
| KH2PO4 | 0.45 | g | 0.45 | 3.307 | |
| (NH4)2SO4 | 0.90 | g | 0.9 | 6.811 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| MgSO4 x 7 H2O | 0.18 | g | 0.18 | 0.73 | |
| CaCl2 x 2 H2O | 0.12 | g | 0.12 | 0.816 | |
|
Starch
(soluble) |
5.00 | g | 5 | - | |
|
Casitone
(Difco) |
10.00 | g | 10 | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| NaHCO3 | 6.00 | g | 6 | 71.423 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. Gas atmosphere: 100% CO2. | |||||
| 2 Distribute into Hungate tubes and autoclave | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Casitone | 10 | - |
| NaHCO3 | 6 | 71.423 |
| Starch | 5 | - |
| NaCl | 0.9 | 15.4 |
| (NH4)2SO4 | 0.9 | 6.811 |
| Show more | ||
