147 .
RUMINOBACTER AMYLOPHILUS MEDIUM
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Equipment needed:
Autoclave
Hungate tubes
Final gas composition:
100 % CO2
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Final pH:
7.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 0.45 | g | ||
|
|
KH2PO4 | 0.45 | g | ||
|
|
(NH4)2SO4 | 0.90 | g | ||
|
|
NaCl | 0.90 | g | ||
|
|
MgSO4 x 7 H2O | 0.18 | g | ||
|
|
CaCl2 x 2 H2O | 0.12 | g | ||
|
|
Starch
(soluble) |
5.00 | g | ||
|
|
Casitone
(Difco) |
10.00 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
NaHCO3 | 6.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.0. Gas atmosphere: 100% CO2. | |||||
| 2 Distribute into Hungate tubes and autoclave | |||||