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Main sol. 147

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Internal Solution-ID: S257

Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.45 g 0.45 2.584
KH2PO4 0.45 g 0.45 3.307
(NH4)2SO4 0.90 g 0.9 6.811
NaCl 0.90 g 0.9 15.4
MgSO4 x 7 H2O 0.18 g 0.18 0.73
CaCl2 x 2 H2O 0.12 g 0.12 0.816
Starch
(soluble) 		5.00 g 5 -
Casitone
(Difco) 		10.00 g 10 -
Resazurin 1.00 mg 1.0e-3 0.004
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
NaHCO3 6.00 g 6 71.423
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0. Gas atmosphere: 100% CO2.
2 Distribute into Hungate tubes and autoclave
147 RUMINOBACTER AMYLOPHILUS MEDIUM