# 147: RUMINOBACTER AMYLOPHILUS MEDIUM


## Main sol. 147

0.45	g	K2HPO4	
0.45	g	KH2PO4	
0.9	g	(NH4)2SO4	
0.9	g	NaCl	
0.18	g	MgSO4 x 7 H2O	
0.12	g	CaCl2 x 2 H2O	
5	g	Starch (soluble)	
10	g	Casitone (Difco)	
1	mg	Resazurin	
0.5	g	L-Cysteine HCl x H2O	
6	g	NaHCO3	
1000	ml	Distilled water	

1. Adjust pH to 7.0. Gas atmosphere: 100% CO2.
2. Distribute into Hungate tubes and autoclave