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DSMZ

DSMZ 144b . CALDICELLULOSIRUPTOR OWENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 144b #

ml
Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.90 g 0.9 16.826
NaCl 0.90 g 0.9 15.4
MgCl2 x 6 H2O 0.10 g 0.1 0.492
CaCl2 x 2 H2O 0.05 g 0.05 0.34
K2HPO4 0.40 g 0.4 2.297
Trace element solution 10.00 ml - -
Yeast extract 3.00 g 3 -
Trypticase peptone
(BD BBL) 		10.00 g 10 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Na2CO3 2.00 g 2 18.87
Wolin's vitamin solution 5.00 ml - -
D-Glucose 5.00 g 5 27.753
Na2S x 9 H2O 1.00 g 1 4.164
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, vitamins, glucose and sulfide), sparge medium with 80% N2 and 20% CO2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Adjust pH of complete medium to 7.2 - 7.4.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium 705).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 0.50 g 0.5 1.487
CoCl2 x 6 H2O 0.15 g 0.15 1.155
MnCl2 x 4 H2O 0.10 g 0.1 0.505
FeSO4 x 7 H2O 0.10 g 0.1 0.36
ZnCl2 0.10 g 0.1 0.734
AlCl3 x 6 H2O 40.00 mg 0.04 0.166
Na2WO4 x 2 H2O 40.00 mg 0.04 0.121
Na2SeO3 x 5 H2O 30.00 mg 0.03 0.114
NiCl2 x 6 H2O 20.00 mg 0.02 0.154
CuCl2 x 2 H2O 20.00 mg 0.02 0.117
H3BO3 10.00 mg 0.01 0.162
Na2MoO4 x 2 H2O 10.00 mg 0.01 0.041
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7 using 2 N NaOH, then dissolve remaining compounds.

Wolin's vitamin solution #

1000
Solution occurs in 54 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 10 -
D-Glucose 5 27.753
Yeast extract 3 -
Na2CO3 2 18.87
Na2S x 9 H2O 1 4.164
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
nutrition chemoorganotroph 1 strain
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