705
705 .
BACILLUS INFERNUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 705 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Ferric citrate monohydrate | 5.3 | g | 5.146 | 19.568 | |
| NaCl | 5.8 | g | 5.631 | 96.356 | |
| NH4Cl | 1.0 | g | 0.971 | 18.151 | |
| K2HPO4 x 3 H2O | 0.4 | g | 0.388 | 2.23 | |
| MgCl2 x 6 H2O | 1.0 | g | 0.971 | 4.776 | |
|
Yeast extract
(OXOID) |
2.0 | g | 1.942 | - | |
|
Trypticase peptone
(BD BBL) |
2.0 | g | 1.942 | - | |
| Trace element solution | 10.0 | ml | - | - | |
| Na-acetate | 0.8 | g | 0.777 | 9.468 | |
| Na-lactate | 1.2 | g | 1.165 | 10.397 | |
|
2-Mercaptoethanesulfonic acid
(coenzyme M) |
0.5 | g | 0.485 | 3.414 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 First, dissolve ferric citrate in water by heating and adjust to pH 7.5 with NaOH, then add other medium ingredients except acetate, lactate, and coenzyme M. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, lactate, and coenzyme M from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
| 2 Prior to inoculation the pH of the complete medium should be checked and adjusted to 7.5, if necessary. | |||||
Solution occurs in 2
other media (first defined in Medium 705).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 0.50 | g | 0.5 | 1.487 | |
| CoCl2 x 6 H2O | 0.15 | g | 0.15 | 1.155 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| AlCl3 x 6 H2O | 40.00 | mg | 0.04 | 0.166 | |
| Na2WO4 x 2 H2O | 40.00 | mg | 0.04 | 0.121 | |
| Na2SeO3 x 5 H2O | 30.00 | mg | 0.03 | 0.114 | |
| NiCl2 x 6 H2O | 20.00 | mg | 0.02 | 0.154 | |
| CuCl2 x 2 H2O | 20.00 | mg | 0.02 | 0.117 | |
| H3BO3 | 10.00 | mg | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 10.00 | mg | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve EDTA in distilled water and adjust pH to 7 using 2 N NaOH, then dissolve remaining compounds. | |||||
Metadata
| Last modified: | 23.05.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 5.631 | 96.356 |
| Ferric citrate monohydrate | 5.146 | 19.568 |
| Yeast extract | 1.942 | - |
| Trypticase peptone | 1.942 | - |
| Na-lactate | 1.165 | 10.397 |
| Show more | ||
