Main sol. 144b
Internal Solution-ID: S252
Equipment needed: Autoclave
Filter
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.90 | g | 0.9 | 16.826 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| K2HPO4 | 0.40 | g | 0.4 | 2.297 | |
| Trace element solution | 10.00 | ml | - | - | |
| Yeast extract | 3.00 | g | 3 | - | |
|
Trypticase peptone
(BD BBL) |
10.00 | g | 10 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 2.00 | g | 2 | 18.87 | |
| Wolin's vitamin solution | 5.00 | ml | - | - | |
| D-Glucose | 5.00 | g | 5 | 27.753 | |
| Na2S x 9 H2O | 1.00 | g | 1 | 4.164 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, vitamins, glucose and sulfide), sparge medium with 80% N2 and 20% CO2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Adjust pH of complete medium to 7.2 - 7.4. | |||||
Media with this solution
| 144b | CALDICELLULOSIRUPTOR OWENSIS MEDIUM |