1328
1328 .
DEFLUVIITOGA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Mesotoga prima DSM 24444
* Modification: Omit Trypticase peptone and fumarate. Increase amount of yeast extract to 2.0 g/l and supplement medium with 0.2 g/l Na-acetate and 0.6 g/l DL-lactate added after autoclaving from sterile anoxic stock solutions prepared under 100% N2 gas.
Growth conditions: 3-7 days
anaerobic
37 °C
Main sol. 1328 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.5 | 2.459 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
|
Yeast extract*
(OXOID) |
2.0 | g | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
|
Sulfur
(powdered) |
10.0 | g | 10 | 311.818 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
|
Trypticase peptone*
(BD BBL) |
2.0 | g | 2 | - | |
| Na2-fumarate* | 3.2 | g | 3.2 | 19.996 | |
| Na2CO3 | 1.0 | g | 1 | 9.435 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Na acetate* | 0.2 | g | - | - | |
| Na-DL-lactate* | 0.6 | g | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, cysteine, peptone, fumarate, carbonate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissove cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at 121°C for 20 min. Add peptone, fumarate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.0. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 25.08.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 10 | 311.818 |
| Na2-fumarate | 3.2 | 19.996 |
| Yeast extract | 2 | - |
| NH4Cl | 1 | 18.695 |
| NaCl | 1 | 17.112 |
| Show more | ||
