Main sol. 1328
Internal Solution-ID: S2663
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.5 | 2.459 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
|
Yeast extract
(OXOID) |
1.0 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
|
Sulfur
(powdered) |
10.0 | g | 10 | 311.818 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
|
Trypticase peptone
(BD BBL) |
2.0 | g | 2 | - | |
| Na2-fumarate | 3.2 | g | 3.2 | 19.996 | |
| Na2CO3 | 1.0 | g | 1 | 9.435 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, cysteine, peptone, fumarate, carbonate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissove cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at 121°C for 20 min. Add peptone, fumarate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.0. | |||||
Media with this solution
| 1328 | DEFLUVIITOGA MEDIUM |