# 1328: DEFLUVIITOGA MEDIUM


## Main sol. 1328

0.3	g	KH2PO4	
0.3	g	K2HPO4	
1	g	NH4Cl	
1	g	NaCl	
0.1	g	KCl	
0.5	g	MgCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
1	ml	**Trace element solution SL-10**	
1	g	Yeast extract (OXOID)	
0.5	ml	Sodium resazurin (0.1% w/v)	
10	g	Sulfur (powdered)	
0.5	g	L-Cysteine HCl x H2O	
2	g	Trypticase peptone (BD BBL)	
3.2	g	Na2-fumarate	
1	g	Na2CO3	
0.5	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients (except sulfur, cysteine, peptone, fumarate, carbonate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissove cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at 121°C for 20 min. Add peptone, fumarate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.0.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.