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DSMZ

Strain modifications for Medium 1328

3 distinct modifications were found.

Omit Trypticase peptone and fumarate. Increase amount of yeast extract to 2.0 g/l and supplement medium with 0.2 g/l Na-acetate and 0.6 g/l DL-lactate added after autoclaving from sterile anoxic stock solutions prepared under 100% N2 gas.

Modified strains

Mesotoga prima DSM 24444

Annotated modifications

Trypticase peptone
Yeast extract
2 g Yeast extract
Na2-fumarate
0.2 g Na acetate
0.6 g Na-DL-lactate

Omit Trypticase peptone and fumarate. Increase amount of yeast extract to 2.0 g/l, supplement medium with 0.2 g/l Na-acetate and add 3.0 g/l D-xylose after autoclaving from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration.

Modified strains

Mesotoga infera DSM 25546

Annotated modifications

0.2 g Na acetate
Trypticase peptone
Yeast extract
2 g Yeast extract
Na2-fumarate
3 g D-Xylose

Supplement medium with 6.0 g/l D-glucose added to the medium after autoclaving from a sterile anoxic stock solution prepared under 100% N2 gas.

Modified strains

Defluviitoga tunisiensis DSM 29926

Annotated modifications

6 g D-Glucose
Modifications
Omit Trypticase peptone and fumarate. Increase amount of yeast extract to 2.0 g/l and supplement medium with 0.2 g/l Na-acetate and 0.6 g/l DL-lactate added after autoclaving from sterile anoxic stock solutions prepared under 100% N2 gas. Omit Trypticase peptone and fumarate. Increase amount of yeast extract to 2.0 g/l, supplement medium with 0.2 g/l Na-acetate and add 3.0 g/l D-xylose after autoclaving from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Supplement medium with 6.0 g/l D-glucose added to the medium after autoclaving from a sterile anoxic stock solution prepared under 100% N2 gas.