DSMZ 1327 . LARKINELLA ARBORICOLA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Larkinella arboricola DSM 21851

* Modification: Adjust pH of final medium to 6.0 - 6.5.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.

Growth conditions: 25 °C
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.1 g 0.1 0.735
N-Acetylglucosamine 1.0 g 1 4.521
Peptone 0.1 g 0.1 -
Yeast extract 0.1 g 0.1 -
Casamino acids 0.1 g 0.1 -
Glucose 0.5 g 0.5 2.775
Hutner's salts 20.0 ml - -
Distilled water 980.0 ml - -
Agar , for solid medium 15.0 g 15 44.599
1 The medium may be solidified by adding 15 g/l agar. Final pH should be 6.0 - 6.5
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 6.0 - 6.5
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Associated strains

All strains for this medium: 1
Strains with modifications: 1
Larkinella arboricola DSM 21851

  6.0 - 6.5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
N-Acetylglucosamine 1 4.521
MgSO4 x 7 H2O 0.594 2.41
Glucose 0.5 2.775
Nitrilotriacetic acid 0.2 1.046
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain