{"medium":{"id":1327,"name":"LARKINELLA ARBORICOLA MEDIUM","complex_medium":"yes","min_pH":6,"max_pH":6.5,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1327.pdf"},"solutions":[{"id":2660,"name":"Main sol. 1327","volume":1000,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.734835,"optional":"no"},{"recipe No.":2,"compound":"N-Acetylglucosamine","compound_id":462,"amount":1,"unit":"g","g_l":1,"mmol_l":4.52059,"optional":"no"},{"recipe No.":3,"compound":"Peptone","compound_id":1,"amount":0.1,"unit":"g","g_l":0.1,"optional":"no"},{"recipe No.":4,"compound":"Yeast extract","compound_id":16,"amount":0.1,"unit":"g","g_l":0.1,"optional":"no"},{"recipe No.":5,"compound":"Casamino acids","compound_id":101,"amount":0.1,"unit":"g","g_l":0.1,"optional":"no"},{"recipe No.":6,"compound":"Glucose","compound_id":5,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.77531,"optional":"no"},{"recipe No.":7,"solution":"Hutner's salts","solution_id":1268,"amount":20,"unit":"ml","optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":980,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Agar","compound_id":3,"condition":"for solid medium","amount":15,"unit":"g","g_l":15,"mmol_l":44.5991,"optional":"no"}],"steps":[{"step":"The medium may be solidified by adding 15 g/l agar. Final pH should be 6.0 - 6.5"}],"main solution":"yes"},{"id":1268,"name":"Hutner's salts","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"attribute":"NTA","amount":10,"unit":"g","g_l":10,"mmol_l":52.3177,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":29.7,"unit":"g","g_l":29.7,"mmol_l":120.499,"optional":"no"},{"recipe No.":3,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":3.335,"unit":"g","g_l":3.335,"mmol_l":22.6848,"optional":"no"},{"recipe No.":4,"compound":"(NH4)6Mo7O24 x 4 H2O","compound_id":120,"amount":9.25,"unit":"mg","g_l":0.00925,"mmol_l":0.00748467,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":99,"unit":"mg","g_l":0.099,"mmol_l":0.356096,"optional":"no"},{"recipe No.":6,"solution":"\"Metals 44\"","solution_id":1269,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":950,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve the nitrilotriacetic acid."},{"step":"Adjust pH to 7.0 with KOH (~7.3 g)."},{"step":"Dissolve other salts separately and combine."},{"step":"Adjust pH to 6.8 with NaOH or H2SO4."}],"Sol. No.":32,"main solution":"no"},{"id":2662,"name":"\"Metals 44\"","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na-EDTA","compound_id":589,"amount":250,"unit":"mg","g_l":0.25,"mmol_l":0.743592,"optional":"no"},{"recipe No.":2,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":1095,"unit":"mg","g_l":1.095,"mmol_l":3.80789,"optional":"no"},{"recipe No.":3,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":500,"unit":"mg","g_l":0.5,"mmol_l":1.79846,"optional":"no"},{"recipe No.":4,"compound":"MnSO4 x H2O","compound_id":14,"amount":154,"unit":"mg","g_l":0.154,"mmol_l":0.911156,"optional":"no"},{"recipe No.":5,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":39.2,"unit":"mg","g_l":0.0392,"mmol_l":0.156997,"optional":"no"},{"recipe No.":6,"compound":"Co(NO3)2 x 6 H2O","compound_id":541,"amount":24.8,"unit":"mg","g_l":0.0248,"mmol_l":0.0852131,"optional":"no"},{"recipe No.":7,"compound":"Na2B4O7 x 10 H2O","compound_id":196,"amount":17.7,"unit":"mg","g_l":0.0177,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions."}],"main solution":"no"},{"id":1269,"name":"\"Metals 44\"","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na-EDTA","compound_id":589,"amount":250,"unit":"mg","g_l":0.25,"mmol_l":0.743592,"optional":"no"},{"recipe No.":2,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":1095,"unit":"mg","g_l":1.095,"mmol_l":3.80789,"optional":"no"},{"recipe No.":3,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":500,"unit":"mg","g_l":0.5,"mmol_l":1.79846,"optional":"no"},{"recipe No.":4,"compound":"MnSO4 x H2O","compound_id":14,"amount":154,"unit":"mg","g_l":0.154,"mmol_l":0.911156,"optional":"no"},{"recipe No.":5,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":39.2,"unit":"mg","g_l":0.0392,"mmol_l":0.156997,"optional":"no"},{"recipe No.":6,"compound":"Co(NO3)2 x 6 H2O","compound_id":541,"amount":24.8,"unit":"mg","g_l":0.0248,"mmol_l":0.0852131,"optional":"no"},{"recipe No.":7,"compound":"Na2B4O7 x 10 H2O","compound_id":196,"amount":17.7,"unit":"mg","g_l":0.0177,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions."}],"Sol. No.":46,"main solution":"no"}]}