1320
1320 .
HALF STRENGTH R2A MEDIUM IN 75% SEAWATER
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1320 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 830 , prepare half strength medium with 75 % seawater | 1000 | ml | - | - | |
| 1 Refer to the composition of DSMZ medium 830 and either make up the medium in 2 litres of 75% seawater or use half of the amounts of the components specified and make up the medium in 1 litre of 75% seawater. Liquid medium can be prepared by leaving out the agar. | |||||
Solution occurs in 8
other media (first defined in Medium 830).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.50 | g | - | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | - | - | |
| Casamino acids | 0.50 | g | - | - | |
| Glucose | 0.50 | g | - | - | |
|
Starch
(soluble) |
0.50 | g | - | - | |
| Na-pyruvate | 0.30 | g | - | - | |
| K2HPO4 | 0.30 | g | - | - | |
| MgSO4 x 7 H2O | 0.05 | g | - | - | |
| Agar | 15.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Metadata
| Last modified: | 15.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.5 | 2.775 |
| Yeast extract | 0.5 | - |
| Proteose peptone | 0.5 | - |
| Casamino acids | 0.5 | - |
| Show more | ||
