1285
1285 .
GRANULICELLA PALUDICOLA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Granulicella acidiphila DSM 28996
* Modification: Add SL-10 (1 ml/L) (see medium 1426)
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 1285 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Fructose | 0.50 | g | 0.5 | 2.775 | |
| Yeast extract | 0.05 | g | 0.05 | - | |
| Casamino acids | 0.05 | g | 0.05 | - | |
| MgSO4 x 7 H2O | 0.04 | g | 0.04 | 0.162 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 | |
| KH2PO4 | 0.10 | g | 0.1 | 0.735 | |
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.757 | |
| Distilled water | 1000.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| Trace element solution SL-10* | 1.00 | ml | - | - | |
| 1 The medium may be solidified with 15 g/l agar. Adjust to pH 4.0 - 5.0 with alginic acid. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 4.0 - 5.0 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Fructose | 0.5 | 2.775 |
| (NH4)2SO4 | 0.1 | 0.757 |
| KH2PO4 | 0.1 | 0.735 |
| Casamino acids | 0.05 | - |
| Show more | ||
