Culture medium recipe
Vol.
×
Main sol. 1191 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-acetate | 0.3 | g | 0.3 | 3.657 | |
| Casamino acids | 0.3 | g | 0.3 | - | |
| Glucose | 0.6 | g | 0.6 | 3.33 | |
| Salt solution | 10.0 | ml | - | - | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Demineralized water | 1000.0 | ml | - | - | |
| 1 Please add after autoclaving: | |||||
| Vitamin solution | 5.0 | ml | - | - | |
| Phosphate solution | 2.0 | ml | - | - | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| (NH4)2SO4 | 1.0 | g | 1 | 7.568 |
| KCl | 0.5 | g | 0.5 | 6.707 |
| MgSO4 x 7 H2O | 0.5 | g | 0.5 | 2.029 |
| CaCl2 x 2 H2O | 0.2 | g | 0.2 | 1.36 |
| CaCO3 | 0.2 | g | 0.2 | 1.998 |
| FeCl3 x 6 H2O | 5.0 | mg | 0.005 | 0.018 |
| Demineralized water | 1000.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 | 2.480 | g | 124 | 873.492 | |
| NaH2PO4 | 0.308 | g | 15.4 | 128.358 | |
| Demineralized water | 20.000 | ml | - | - | |
| 1 Adjust pH to 7.2 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Riboflavin | 10.0 | mg | 0.1 | 0.266 |
| Thiamine-HCl x 2 H2O | 50.0 | mg | 0.5 | 1.339 |
| Nicotinic acid | 50.0 | mg | 0.5 | 4.061 |
| Pyridoxine hydrochloride | 50.0 | mg | 0.5 | 2.431 |
| Calcium pantothenate | 50.0 | mg | 0.5 | 1.049 |
| Biotin | 0.1 | mg | 1.0e-3 | 0.004 |
| Folic acid | 0.2 | mg | 0.002 | 0.005 |
| Vitamin B12 | 1.0 | mg | 0.01 | 0.007 |
| Demineralized water | 100.0 | ml | - | - |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.6 | 3.33 |
| Na-acetate | 0.3 | 3.657 |
| Casamino acids | 0.3 | - |
| Na2HPO4 | 0.248 | 1.747 |
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