1191 .
EGGC
What is this page?
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na-acetate | 0.3 | g | ||
|
|
Casamino acids | 0.3 | g | ||
|
|
Glucose | 0.6 | g | ||
|
|
Salt solution | 10.0 | ml | ||
|
|
Agar | 15.0 | g | ||
|
|
Demineralized water | 1000.0 | ml | ||
| 1 Please add after autoclaving: | |||||
|
|
Vitamin solution | 5.0 | ml | ||
|
|
Phosphate solution | 2.0 | ml | ||
Salt solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 1.0 | g |
|
|
KCl | 0.5 | g |
|
|
MgSO4 x 7 H2O | 0.5 | g |
|
|
CaCl2 x 2 H2O | 0.2 | g |
|
|
CaCO3 | 0.2 | g |
|
|
FeCl3 x 6 H2O | 5.0 | mg |
|
|
Demineralized water | 1000.0 | ml |
Phosphate solution #
20
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 | 2.480 | g | ||
|
|
NaH2PO4 | 0.308 | g | ||
|
|
Demineralized water | 20.000 | ml | ||
| 1 Adjust pH to 7.2 | |||||
Vitamin solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Riboflavin | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 50.0 | mg |
|
|
Nicotinic acid | 50.0 | mg |
|
|
Pyridoxine hydrochloride | 50.0 | mg |
|
|
Calcium pantothenate | 50.0 | mg |
|
|
Biotin | 0.1 | mg |
|
|
Folic acid | 0.2 | mg |
|
|
Vitamin B12 | 1.0 | mg |
|
|
Demineralized water | 100.0 | ml |