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DSMZ

DSMZ 1148 . TINDALLIA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1148 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
KCl 0.20 g 0.2 2.683
K2HPO4 0.20 g 0.2 1.148
MgCl2 x 6 H2O 0.10 g 0.1 0.492
NH4Cl 1.00 g 1 18.695
Yeast extract 0.50 g 0.5 -
Peptone 5.00 g 5 -
Trace element solution (mg per 200 ml) 1.00 ml - -
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Prepare the medium omitting Na2CO3, NaHCO3, Na2S x 9 H2O, and Vitamin solution.
2 Boil the medium and cool under nitrogen, add Na2CO3 and NaHCO3.
NaHCO3 24.00 g 24 285.69
Na2CO3 2.76 g 2.76 26.041
3 Adjust pH to 9.5-10.0 with 10M KOH.
4 Dispense into tubes or bottle fitted with rubber stoppers.
5 Autoclave at 121 °C for 15 min.
6 Add Na2S x 9 H2O (neutralized) and Vitamin solution from sterile stock solutions
Na2S x 9 H2O
(from neutralized stock solution) 		0.40 g 0.4 1.665
Wolin's vitamin solution 2.00 ml - -

Trace element solution (mg per 200 ml) #

200
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 720 mg 3.6 18.191
Fe(NH4)2(SO4)2 x 12 H2O 400 mg 2 -
FeSO4 x 7 H2O 200 mg 1 3.597
CoCl2 x 6 H2O 200 mg 1 7.702
ZnSO4 x 7 H2O 200 mg 1 3.478
Na2MoO4 x 2 H2O 20 mg 0.1 0.413
NiCl2 100 mg 0.5 3.858
CuSO4 x 5 H2O 20 mg 0.1 0.401
AlK(SO4)2 x 12 H2O 20 mg 0.1 0.211
H3BO3 20 mg 0.1 1.617
HCl
(concentrated) 		5 ml - -
Distilled water 200 ml - -

Wolin's vitamin solution #

1000
Solution occurs in 54 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 03.02.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5 - 10.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 24 285.69
Peptone 5 -
Na2CO3 2.76 26.041
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain