Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 30 | 513.347 | |
| KCl | 0.20 | g | 0.2 | 2.683 | |
| K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| Peptone | 5.00 | g | 5 | - | |
| Trace element solution (mg per 200 ml) | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium omitting Na2CO3, NaHCO3, Na2S x 9 H2O, and Vitamin solution. | |||||
| 2 Boil the medium and cool under nitrogen, add Na2CO3 and NaHCO3. | |||||
| NaHCO3 | 24.00 | g | 24 | 285.69 | |
| Na2CO3 | 2.76 | g | 2.76 | 26.041 | |
| 3 Adjust pH to 9.5-10.0 with 10M KOH. | |||||
| 4 Dispense into tubes or bottle fitted with rubber stoppers. | |||||
| 5 Autoclave at 121 °C for 15 min. | |||||
| 6 Add Na2S x 9 H2O (neutralized) and Vitamin solution from sterile stock solutions | |||||
|
Na2S x 9 H2O
(from neutralized stock solution) |
0.40 | g | 0.4 | 1.665 | |
| Wolin's vitamin solution | 2.00 | ml | - | - | |
Media with this solution
| 1148 | TINDALLIA MEDIUM |