Main sol. 371
Internal Solution-ID: S711
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| KCl | 1.00 | g | 1 | 13.414 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgSO4 x 7 H2O | 0.24 | g | 0.24 | 0.974 | |
| CaSO4 x 2 H2O | 0.17 | g | 0.17 | 0.987 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Agar , if necessary | 20.00 | g | 20 | 59.465 | |
| NaCl | 200.00 | g | 200 | 3422.31 | |
| Na2-glutamate | 1.00 | g | 1 | 5.913 | |
|
Yeast extract
(Fisher BioReagents BP1422) |
5.00 | g | 5 | - | |
| Casamino acids | 5.00 | g | 5 | - | |
| Na2CO3 | 5.00 | g | 5 | 47.175 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add distilled water to give a final volume of 1000 ml. | |||||
| 2 Adjust pH to 6.5 before autoclaving! | |||||
| 3 Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5. | |||||
| 4 If agar medium is prepared, heat and dissolve agar before adding sodium chloride. | |||||
Media with this solution
| 371 | NATRONOBACTERIA MEDIUM |